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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Corte. Para informações adicionais entre em contato com cnpgc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
23/01/2013 |
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Data da última atualização: |
15/02/2013 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOUSA, A. C. B.; CARVALHO, M. A.; CAMPOS, T.; ZUCCHI, M. I.; JANK, L.; SOUZA, A. P. |
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Afiliação: |
Vegetal Biology Department (PPGBVeg), Universidade do Estado da Bahia (UNEB), Campus VIII, Rua do Bom Conselho, 179, Paulo Afonso, BA; Brazilian Agricultural Research Corporation, Embrapa Cerrados, BR 020, km 18., Planaltina, DF CEP; Brazilian Agricultural Research Corporation, Embrapa Acre, BR 364, km 14, Rio Branco, AC; São Paulo Agency of Technology and Agro-Business, Po´lo Apta Centro Sul, Rod. SP 127 km 30, CP 28, Piracicaba, SP; LIANA JANK, CNPGC; Plant Biology Department (DBV), Biology Institute, University of Campinas (UNICAMP), CP6109, Campinas, SP. |
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Título: |
Molecular diversity, genetic structure and mating system of Calopogonium mucunoides Desv. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Genetic Resources and Crop Evolution, v.59, n.8, p.1449-1464, 2012. |
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Idioma: |
Inglês |
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Conteúdo: |
Calopogonium mucunoides Desv. is a species native of South and Central America that is used as green manure and a pasture crop. The molecular genetic diversity was characterized in 195 C. mucunoides accessions from a germplasm collection using 17 microsatellite markers. Outcrossing rate was estimated after the evaluation of six microsatellite loci in 200 genotypes originated from 10 open-pollinated progenies (20 genotypes per progeny). Six genetic clusters were identified in the germplasm collection by the STRUCTURE software analysis, neighbor-joining tree comparisons and principal component analysis, which highly correlated with the geographic locations where these accessions were originated or collected. These results were confirmed using AMOVA. The largest portion of the genetic variation was observed among clusters (64.38%). The results indicated that: multilocus outcrossing rate (t m ) was 16.3%, suggesting a mixed mating system with a predominance of autogamy; single locus outcrossing rate (t s ) was 11%; difference (t m ?t s ) was 0.054, indicating that only 5.4% of outcrossing occurred among related individuals; paternity correlation (r p ) was 33% suggesting a low probability of finding full sibs among the progeny; parental coefficient of inbreeding (F m ) was 5.0%, indicating a low degree of inbreeding in each parent. A core collection for C. mucunoides was assembled to capture the allelic diversity found in this study. The complete allelic diversity was represented by only 15 accessions. These results should be useful for exploiting the genetic resources of C. mucunoides and could influence future conservation efforts and breeding programs. MenosCalopogonium mucunoides Desv. is a species native of South and Central America that is used as green manure and a pasture crop. The molecular genetic diversity was characterized in 195 C. mucunoides accessions from a germplasm collection using 17 microsatellite markers. Outcrossing rate was estimated after the evaluation of six microsatellite loci in 200 genotypes originated from 10 open-pollinated progenies (20 genotypes per progeny). Six genetic clusters were identified in the germplasm collection by the STRUCTURE software analysis, neighbor-joining tree comparisons and principal component analysis, which highly correlated with the geographic locations where these accessions were originated or collected. These results were confirmed using AMOVA. The largest portion of the genetic variation was observed among clusters (64.38%). The results indicated that: multilocus outcrossing rate (t m ) was 16.3%, suggesting a mixed mating system with a predominance of autogamy; single locus outcrossing rate (t s ) was 11%; difference (t m ?t s ) was 0.054, indicating that only 5.4% of outcrossing occurred among related individuals; paternity correlation (r p ) was 33% suggesting a low probability of finding full sibs among the progeny; parental coefficient of inbreeding (F m ) was 5.0%, indicating a low degree of inbreeding in each parent. A core collection for C. mucunoides was assembled to capture the allelic diversity found in this study. The complete allelic diversity was represente... Mostrar Tudo |
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Palavras-Chave: |
Allelic diversity; Germplasm collection; Microsatellite markers; Outcrossing rate. |
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Thesagro: |
Calopogonium mucunoides. |
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Thesaurus Nal: |
autogamy. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02435naa a2200253 a 4500
001 1946128
005 2013-02-15
008 2012 bl uuuu u00u1 u #d
100 1 $aSOUSA, A. C. B.
245 $aMolecular diversity, genetic structure and mating system of Calopogonium mucunoides Desv.$h[electronic resource]
260 $c2012
520 $aCalopogonium mucunoides Desv. is a species native of South and Central America that is used as green manure and a pasture crop. The molecular genetic diversity was characterized in 195 C. mucunoides accessions from a germplasm collection using 17 microsatellite markers. Outcrossing rate was estimated after the evaluation of six microsatellite loci in 200 genotypes originated from 10 open-pollinated progenies (20 genotypes per progeny). Six genetic clusters were identified in the germplasm collection by the STRUCTURE software analysis, neighbor-joining tree comparisons and principal component analysis, which highly correlated with the geographic locations where these accessions were originated or collected. These results were confirmed using AMOVA. The largest portion of the genetic variation was observed among clusters (64.38%). The results indicated that: multilocus outcrossing rate (t m ) was 16.3%, suggesting a mixed mating system with a predominance of autogamy; single locus outcrossing rate (t s ) was 11%; difference (t m ?t s ) was 0.054, indicating that only 5.4% of outcrossing occurred among related individuals; paternity correlation (r p ) was 33% suggesting a low probability of finding full sibs among the progeny; parental coefficient of inbreeding (F m ) was 5.0%, indicating a low degree of inbreeding in each parent. A core collection for C. mucunoides was assembled to capture the allelic diversity found in this study. The complete allelic diversity was represented by only 15 accessions. These results should be useful for exploiting the genetic resources of C. mucunoides and could influence future conservation efforts and breeding programs.
650 $aautogamy
650 $aCalopogonium mucunoides
653 $aAllelic diversity
653 $aGermplasm collection
653 $aMicrosatellite markers
653 $aOutcrossing rate
700 1 $aCARVALHO, M. A.
700 1 $aCAMPOS, T.
700 1 $aZUCCHI, M. I.
700 1 $aJANK, L.
700 1 $aSOUZA, A. P.
773 $tGenetic Resources and Crop Evolution$gv.59, n.8, p.1449-1464, 2012.
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Registro original: |
Embrapa Gado de Corte (CNPGC) |
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Biblioteca |
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Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
28/01/2011 |
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Data da última atualização: |
19/05/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 4 |
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Autoria: |
VALICENTE, F. H.; TUELHER, E. de S.; LEITE, M. I. S.; FREIRE, F. L.; VIEIRA, C. M. |
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Afiliação: |
FERNANDO HERCOS VALICENTE, CNPMS. |
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Título: |
Production of Bacillus thuringiensis biopesticide using commercial lab medium and agricultural by-products as nutrient sources. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Revista Brasileira de Milho e Sorgo, Sete Lagoas, v. 9, n. 1, p. 1-11, 2010. |
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DOI: |
http://dx.doi.org/10.18512/1980-6477/rbms.v9n1p1-11 |
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Idioma: |
Inglês |
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Conteúdo: |
Bacillus thuringiensis (Bt) is a Gram-positive bacterium naturally found in soil, water and grain dust, and can be cultivated in liquid, solid and semi-solid media. The objective of this work was to test different media to grow B. thuringiensis. The seed culture (strain 344, B. thuringiensis tolworthi, belonging to Embrapa Maize and Sorghum Microorganism Bank) was produced using shake flasks and grown in LB medium plus salts during 18 hours, incubated on a rotary shaker at 200 revolutions per minute (rpm) at 30oC for 96 hours. Medium 1 was composed of: Luria Bertani (LB) plus salts (FeSO4, ZnSO4, MnSO4, MgSO4), and 0.2% glucose; medium 2 was composed of 1.5% glucose, 0.5% soybean flour plus salts; and medium 3 was composed of liquid swine manure at 4% and 0.2% glucose. All three media were sterilized and inoculated with B. thuringiensis tolwothi (seed culture) atastirrer speed of 200rpm, for 96 hours at 30oC. The pH was measured at regular intervals, viable spores were counted as c.f.u/mL, cell mass expressed in g/L- lyophilized, and spore counting per mL of medium. All three media showed pH variation during the fermentation process. Media 1 and 2 showed a tendency to shift toward a basic pH and medium 3 to an acidic pH. Media 1 and 2 showed the highest number of viable spores, 2.0 x 108 c.f.u/mL, within the 96 hours of incubation, however medium 2 showed a biomass dry weight of 1.18g/L. During the fermentation period, medium 1 showed the highest spore concentration, 1.4 x 109 spores/mL after 96h of fermentation. Efficiency against S. frugiperda first instar larvae showed that all Bt produced in all three media killed above 60% in the highest concentrations. MenosBacillus thuringiensis (Bt) is a Gram-positive bacterium naturally found in soil, water and grain dust, and can be cultivated in liquid, solid and semi-solid media. The objective of this work was to test different media to grow B. thuringiensis. The seed culture (strain 344, B. thuringiensis tolworthi, belonging to Embrapa Maize and Sorghum Microorganism Bank) was produced using shake flasks and grown in LB medium plus salts during 18 hours, incubated on a rotary shaker at 200 revolutions per minute (rpm) at 30oC for 96 hours. Medium 1 was composed of: Luria Bertani (LB) plus salts (FeSO4, ZnSO4, MnSO4, MgSO4), and 0.2% glucose; medium 2 was composed of 1.5% glucose, 0.5% soybean flour plus salts; and medium 3 was composed of liquid swine manure at 4% and 0.2% glucose. All three media were sterilized and inoculated with B. thuringiensis tolwothi (seed culture) atastirrer speed of 200rpm, for 96 hours at 30oC. The pH was measured at regular intervals, viable spores were counted as c.f.u/mL, cell mass expressed in g/L- lyophilized, and spore counting per mL of medium. All three media showed pH variation during the fermentation process. Media 1 and 2 showed a tendency to shift toward a basic pH and medium 3 to an acidic pH. Media 1 and 2 showed the highest number of viable spores, 2.0 x 108 c.f.u/mL, within the 96 hours of incubation, however medium 2 showed a biomass dry weight of 1.18g/L. During the fermentation period, medium 1 showed the highest spore concentration, 1.4 x 1... Mostrar Tudo |
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Palavras-Chave: |
Biocontrole; Inseticida microbiano. |
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Thesagro: |
Inseticida; Praga de planta; Spodoptera Frugiperda. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/26116/1/Valicente.pdf
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Marc: |
LEADER 02501naa a2200241 a 4500
001 1874936
005 2017-05-19
008 2010 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.18512/1980-6477/rbms.v9n1p1-11$2DOI
100 1 $aVALICENTE, F. H.
245 $aProduction of Bacillus thuringiensis biopesticide using commercial lab medium and agricultural by-products as nutrient sources.$h[electronic resource]
260 $c2010
520 $aBacillus thuringiensis (Bt) is a Gram-positive bacterium naturally found in soil, water and grain dust, and can be cultivated in liquid, solid and semi-solid media. The objective of this work was to test different media to grow B. thuringiensis. The seed culture (strain 344, B. thuringiensis tolworthi, belonging to Embrapa Maize and Sorghum Microorganism Bank) was produced using shake flasks and grown in LB medium plus salts during 18 hours, incubated on a rotary shaker at 200 revolutions per minute (rpm) at 30oC for 96 hours. Medium 1 was composed of: Luria Bertani (LB) plus salts (FeSO4, ZnSO4, MnSO4, MgSO4), and 0.2% glucose; medium 2 was composed of 1.5% glucose, 0.5% soybean flour plus salts; and medium 3 was composed of liquid swine manure at 4% and 0.2% glucose. All three media were sterilized and inoculated with B. thuringiensis tolwothi (seed culture) atastirrer speed of 200rpm, for 96 hours at 30oC. The pH was measured at regular intervals, viable spores were counted as c.f.u/mL, cell mass expressed in g/L- lyophilized, and spore counting per mL of medium. All three media showed pH variation during the fermentation process. Media 1 and 2 showed a tendency to shift toward a basic pH and medium 3 to an acidic pH. Media 1 and 2 showed the highest number of viable spores, 2.0 x 108 c.f.u/mL, within the 96 hours of incubation, however medium 2 showed a biomass dry weight of 1.18g/L. During the fermentation period, medium 1 showed the highest spore concentration, 1.4 x 109 spores/mL after 96h of fermentation. Efficiency against S. frugiperda first instar larvae showed that all Bt produced in all three media killed above 60% in the highest concentrations.
650 $aInseticida
650 $aPraga de planta
650 $aSpodoptera Frugiperda
653 $aBiocontrole
653 $aInseticida microbiano
700 1 $aTUELHER, E. de S.
700 1 $aLEITE, M. I. S.
700 1 $aFREIRE, F. L.
700 1 $aVIEIRA, C. M.
773 $tRevista Brasileira de Milho e Sorgo, Sete Lagoas$gv. 9, n. 1, p. 1-11, 2010.
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Embrapa Milho e Sorgo (CNPMS) |
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